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Structured Review

Difco gram stain kit
Gram Stain Kit, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gram-stain+kit/gram+kit+s+set+stain/pm41857405-60-10-13
Average 86 stars, based on 1 article reviews
gram stain kit - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Cell Culture:

Article Title: Flavobacterium sangjuense sp. nov. isolated from sediment.
Article Snippet: A yellow-pigmented bacterial strain, GS03, was isolated from sediment in a branch of the Nackdong River in Sangju, Korea.. Cells were observed to be Gram-negative, aerobic and rod-shaped with gliding motility, and to be positive for catalase and oxidase.. Growth was found to occur at 4–30 C (optimum 25 C), at pH 7.0–8.5 (optimum pH 7.5) and at NaCl 0% (optimum NaCl 0%, w/v).

Article Title: Description of Paenibacillus jiagnxiensis sp. nov., a mesotrione-degrading bacterium isolated from farmland and cloning of a novel mesotrione nitroreductase gene.
Article Snippet: clade within the genus Paenibacillus.. The major cellular fatty acids were anteiso-C15:0, C16:0, anteisoC17:0 and iso-C16:0, the predominant menaquinone was menaquinone-7 (MK-7), the polar lipids profile consisted of phosphatidyl ethanolamine (PE), phosphatidyl monomethyl ethanolamine (PME), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), one unidentified aminolipid (AL), one unidentified aminophospholipid (APL), two unidentified glycolipids (GL1-2), two unidentified phospholipids (PL1-2), and two unidentified polar lipids (L1-2).. The DNA G + C content was 49.9%.

Article Title: Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
Article Snippet: An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulosedegrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.. The strain was Grampositive, non-endospore forming, non-motile, and formed orange colonies on solid medium.. Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).

Article Title: Collinsella acetigenes sp. nov., an Anaerobic Actinobacterium Isolated from Human Feces, and Emended Description of the Genus Collinsella and Collinsella aerofaciens.
Article Snippet: A novel actinobacterial strain, Gram-positive, anaerobic, non-motile, and rod-shaped, designated KGMB02528T, was isolated from healthy human feces.. Cells of strain KGMB02528T grew optimally at pH 7.0 and 37 °C and in the presence of 0% (w/v) NaCl.. Based on 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Collinsella and was most closely related to Collinsella aerofaciens DSM 17552T (95.8%).

Article Title: Ephemeroptericola cinctiostellae gen. nov., sp. nov., isolated from gut of an aquatic insect.
Article Snippet: The Gram reaction was determined using a Gram-Stain Kit (Difco) according to the manufacturer’s instructions.

Article Title: Bacteroides faecalis sp. nov., isolated from human faeces.
Article Snippet: Gram-staining was determined by using a Gram-stain kit (Difco) according to the manufacturer’s instructions.

Staining:

Article Title: Flavobacterium sangjuense sp. nov. isolated from sediment.
Article Snippet: A yellow-pigmented bacterial strain, GS03, was isolated from sediment in a branch of the Nackdong River in Sangju, Korea.. Cells were observed to be Gram-negative, aerobic and rod-shaped with gliding motility, and to be positive for catalase and oxidase.. Growth was found to occur at 4–30 C (optimum 25 C), at pH 7.0–8.5 (optimum pH 7.5) and at NaCl 0% (optimum NaCl 0%, w/v).

Article Title: Description of Paenibacillus jiagnxiensis sp. nov., a mesotrione-degrading bacterium isolated from farmland and cloning of a novel mesotrione nitroreductase gene.
Article Snippet: clade within the genus Paenibacillus.. The major cellular fatty acids were anteiso-C15:0, C16:0, anteisoC17:0 and iso-C16:0, the predominant menaquinone was menaquinone-7 (MK-7), the polar lipids profile consisted of phosphatidyl ethanolamine (PE), phosphatidyl monomethyl ethanolamine (PME), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), one unidentified aminolipid (AL), one unidentified aminophospholipid (APL), two unidentified glycolipids (GL1-2), two unidentified phospholipids (PL1-2), and two unidentified polar lipids (L1-2).. The DNA G + C content was 49.9%.

Article Title: Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
Article Snippet: An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulosedegrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.. The strain was Grampositive, non-endospore forming, non-motile, and formed orange colonies on solid medium.. Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).

Article Title: Collinsella acetigenes sp. nov., an Anaerobic Actinobacterium Isolated from Human Feces, and Emended Description of the Genus Collinsella and Collinsella aerofaciens.
Article Snippet: A novel actinobacterial strain, Gram-positive, anaerobic, non-motile, and rod-shaped, designated KGMB02528T, was isolated from healthy human feces.. Cells of strain KGMB02528T grew optimally at pH 7.0 and 37 °C and in the presence of 0% (w/v) NaCl.. Based on 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Collinsella and was most closely related to Collinsella aerofaciens DSM 17552T (95.8%).

Article Title: Ephemeroptericola cinctiostellae gen. nov., sp. nov., isolated from gut of an aquatic insect.
Article Snippet: The Gram reaction was determined using a Gram-Stain Kit (Difco) according to the manufacturer’s instructions.

Article Title: Bacteroides faecalis sp. nov., isolated from human faeces.
Article Snippet: Gram-staining was determined by using a Gram-stain kit (Difco) according to the manufacturer’s instructions.

Microscopy:

Article Title: Flavobacterium sangjuense sp. nov. isolated from sediment.
Article Snippet: A yellow-pigmented bacterial strain, GS03, was isolated from sediment in a branch of the Nackdong River in Sangju, Korea.. Cells were observed to be Gram-negative, aerobic and rod-shaped with gliding motility, and to be positive for catalase and oxidase.. Growth was found to occur at 4–30 C (optimum 25 C), at pH 7.0–8.5 (optimum pH 7.5) and at NaCl 0% (optimum NaCl 0%, w/v).

Article Title: Description of Paenibacillus jiagnxiensis sp. nov., a mesotrione-degrading bacterium isolated from farmland and cloning of a novel mesotrione nitroreductase gene.
Article Snippet: clade within the genus Paenibacillus.. The major cellular fatty acids were anteiso-C15:0, C16:0, anteisoC17:0 and iso-C16:0, the predominant menaquinone was menaquinone-7 (MK-7), the polar lipids profile consisted of phosphatidyl ethanolamine (PE), phosphatidyl monomethyl ethanolamine (PME), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), one unidentified aminolipid (AL), one unidentified aminophospholipid (APL), two unidentified glycolipids (GL1-2), two unidentified phospholipids (PL1-2), and two unidentified polar lipids (L1-2).. The DNA G + C content was 49.9%.

Article Title: Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
Article Snippet: An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulosedegrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.. The strain was Grampositive, non-endospore forming, non-motile, and formed orange colonies on solid medium.. Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).

Article Title: Collinsella acetigenes sp. nov., an Anaerobic Actinobacterium Isolated from Human Feces, and Emended Description of the Genus Collinsella and Collinsella aerofaciens.
Article Snippet: A novel actinobacterial strain, Gram-positive, anaerobic, non-motile, and rod-shaped, designated KGMB02528T, was isolated from healthy human feces.. Cells of strain KGMB02528T grew optimally at pH 7.0 and 37 °C and in the presence of 0% (w/v) NaCl.. Based on 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Collinsella and was most closely related to Collinsella aerofaciens DSM 17552T (95.8%).

Article Title: Ephemeroptericola cinctiostellae gen. nov., sp. nov., isolated from gut of an aquatic insect.
Article Snippet: The Gram reaction was determined using a Gram-Stain Kit (Difco) according to the manufacturer’s instructions.

Article Title: Bacteroides faecalis sp. nov., isolated from human faeces.
Article Snippet: Gram-staining was determined by using a Gram-stain kit (Difco) according to the manufacturer’s instructions.

Transmission Assay:

Article Title: Flavobacterium sangjuense sp. nov. isolated from sediment.
Article Snippet: A yellow-pigmented bacterial strain, GS03, was isolated from sediment in a branch of the Nackdong River in Sangju, Korea.. Cells were observed to be Gram-negative, aerobic and rod-shaped with gliding motility, and to be positive for catalase and oxidase.. Growth was found to occur at 4–30 C (optimum 25 C), at pH 7.0–8.5 (optimum pH 7.5) and at NaCl 0% (optimum NaCl 0%, w/v).

Article Title: Description of Paenibacillus jiagnxiensis sp. nov., a mesotrione-degrading bacterium isolated from farmland and cloning of a novel mesotrione nitroreductase gene.
Article Snippet: clade within the genus Paenibacillus.. The major cellular fatty acids were anteiso-C15:0, C16:0, anteisoC17:0 and iso-C16:0, the predominant menaquinone was menaquinone-7 (MK-7), the polar lipids profile consisted of phosphatidyl ethanolamine (PE), phosphatidyl monomethyl ethanolamine (PME), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), one unidentified aminolipid (AL), one unidentified aminophospholipid (APL), two unidentified glycolipids (GL1-2), two unidentified phospholipids (PL1-2), and two unidentified polar lipids (L1-2).. The DNA G + C content was 49.9%.

Article Title: Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium.
Article Snippet: An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulosedegrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source.. The strain was Grampositive, non-endospore forming, non-motile, and formed orange colonies on solid medium.. Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T).

Article Title: Collinsella acetigenes sp. nov., an Anaerobic Actinobacterium Isolated from Human Feces, and Emended Description of the Genus Collinsella and Collinsella aerofaciens.
Article Snippet: A novel actinobacterial strain, Gram-positive, anaerobic, non-motile, and rod-shaped, designated KGMB02528T, was isolated from healthy human feces.. Cells of strain KGMB02528T grew optimally at pH 7.0 and 37 °C and in the presence of 0% (w/v) NaCl.. Based on 16S rRNA gene sequence similarity, strain KGMB04489T belonged to the genus Collinsella and was most closely related to Collinsella aerofaciens DSM 17552T (95.8%).

Article Title: Ephemeroptericola cinctiostellae gen. nov., sp. nov., isolated from gut of an aquatic insect.
Article Snippet: The Gram reaction was determined using a Gram-Stain Kit (Difco) according to the manufacturer’s instructions.

Article Title: Bacteroides faecalis sp. nov., isolated from human faeces.
Article Snippet: Gram-staining was determined by using a Gram-stain kit (Difco) according to the manufacturer’s instructions.



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